Active Air Sampling Sop

This leaves determination of the number ofsites for passive air sampling and surface sampling.

Active air sampling sop.

Place the axis in vertical position. Active air sampling requires the use of a microbiology air sampler to physically draw a known volume of air over or through a particle collection device and there are two main types. This nut will be the bottom limit. Sterilise the stainless steel aspirating head of the air sampler prior to use by autoclaving as per sop.

Carry the preincubated scda plates and sterile ss head in a ss container to the sampling location. 6 1 6 place the cassette in the air sampler under an laf. The organisms are then visible to the naked eyes and can be counted for an assessment of the level of contamination. Analyze the microbial quality of air by sampling 100 litres of air at each of the locations mentioned in annexure i using active air sampler carry the pre incubated scda plates and sterile ss head in a ss container to the sampling location.

In active monitoring a microbial air sampler is used to force air into or onto its collection medium e g petri dish with nutrient agar based test media over a specified period of time. When the pre set sampling cycle is completed the plate is removed and incubated. 5 9 6 ensure that m air t media cassette is in place before sampling is started. At 3 cm of the end of the axis screw a nut.

6 2 1 push the on off button. Airborne gases and vapours are collected by a physical process such as diffusion through a static air layer or permeation through a membrane. The collected culture can then be incubated and. The following steps describe the way to assemble the passive air sampler.

Enter into the aseptic area following aseptic area entry and exit sop of the concerned block. Passive air sampling passive air sampling i e settle plates is a frequently. Active there are two primary methods for microbial air sampling. Fertility test of media.

Typically sampled air is drawn by a suction pump through a narrow inlet tube into a small. 1 impingers impingers use a liquid medium for particle collection. Active sampling involves the use of an air sampling pump to actively pull air through a collection device such as a filter. Active air sampling sites should be done at the same locations or as close as practical to avoid compro mising the other measure or the product integrity.

6 2 switching the sampler on. 5 9 5 regulate the pressure of the air so that the flow rate is in between 140l to 180l per minute. 5 2 operation of air. Active and passive monitoring.

5 9 7 close the media cassettes properly after sampling 5 9 8 clean the pump with 70 ipa before taking it to the next sampling location port. Standard operating procedures for the passive air sampling 2 2 pas assembling the pas should be assembled from the bottom to the top. 6 2 2 after pressing the on off button the previously selected air sample volume retained in the memory appears on the display.